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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
, gv3101 - 25GEN 2. Transfer O/N culture to 200ml YEP in a sterile 500ml flask and shake at 250rpm until the OD is 0.3 (4-5hrs) 3. Spin in sterile 50ml screw cap tubes 4C 5krpm 10‚. Check to make sure cells are pelleted, if not repeat at higher
" Solutions. 1. A molar solution is one in which 1 liter of solution contains the number of grams equal to its molecular weight. Ex. To make up 100 ml of a 5M NaCl solution = 58.456 (mw of NaCl) g x 5 moles x 0.1 liter = 29.29 g in 100 ml
interfering RNAs for specific loss-of-function studies. Nat. Methods 4: 337-344.[Medline] Copyright © 2007 by Cold Spring Harbor Laboratory Press. Online ISSN: 1559-6095 Terms of Service All rights reserved. Anyone using the procedures
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