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文献和实验Lipoprotein Isolation - First week
of a tissue. 14. Place the metal cap of the tubes, and seal with the heat sealer. As soon as the cap reaches the shoulder of the tube, quicly move the tube to the right and push the cap down with the heat sink. 15. Place the four tubes in the VTi
Lipoprotein Isolation - First week
needle and a capillary tubing (see picture ). 10. Let the hemolymph flow into the heat sealable centrifuge tube. 11. Add another 20 ml PBS into the syringe and overlay on the hemolymph. 12. Turn on the heat sealer to warm it up. 13
are precipitated with sodium acetate, and the lysate is cleared first by filtration of precipitate through cheesecloth and then by centrifugation. The DNA-containing supernatant is transferred to a new tube, and the plasmid or cosmid DNA is precipitated
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