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- 2025年07月09日
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文献和实验Partial proteolysis with Staph. aureus V8 protease
in the wells of the gel, using the teflon tamper to push them down to the bottom. Add 10 microliters of the Rehydration/Overlay buffer. This will seal any space between the gel pieces and the wells. Add the desired amounts of protease (10, 50, 200 ng
Yeast IF with Methanol/Acetone Dehydration
the wells of teflon coated slides with 0.1 polylysine (>400,000 MW) in water for 10 min at RT. Spin this solution for 10 min in a microfuge immedietly before use and stay away from the bottom. In general do high speed spins of all solutions
Yeast IF with Methanol/Acetone Dehydration
part of the procedure and unfortunately it is the most critical as well. Wash the cells once in sorbitol buffer and suspend in 100-500ul of the same. BE VERY VERY GENTLE! Place on ice. Coat the wells of teflon coated slides with 0.1% polylysine (>400,000 MW
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