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文献和实验that carrying the mutation is not the same thing as having the disease. Unfortunately, there are still many situations where direct mutation tests are not available as part of a routine diagnostic service. Some disease genes have not yet been cloned
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
3) Gently mix and zing. Add primer mix to reaction mix (final volume 20ul) [primer mix now actually about 7ul] Mix and incubate at 42°C for 2 hours Stop reaction with 3.5ul of 0.5M NaOH, 50mM EDTA [Probably should make fresh] Incubate at 65°C
Basic Analysis of NimbleGen ChIP-on-chip Data using Bioconductor/R
Introduction Hybridization of chromatin immuno-precipitation (ChIP) material to tiling arrays at NimbleGen service facilities usually leaves the customer with a set of data files that are of limited use. Most information about the experiment
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