- ￥3591
- Beckman Coulter贝克曼库尔特
- 美国
- B90300
- 2025年07月14日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海博耀商贸有限公司
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Ab concentrations.OD (Absorbance at 280 nm)/1.35 = X mg/ml.Store Abs at -20℃ or further concentrate by using Centriprep. And store at -20℃.Regenerate Ab column with Regenerate buffer (~200 ml).
Bacterial Expression of IRS-1 containing GST-fusion Proteins
1. Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture. 2. Grow larger culture using the overnight culture as a seeding culture. The culture is grown in LB-Amp medium. Aerate well with shaking at 37 C until OD at 600nm
(collect at ~5 min/fraction). 25 tubes are sufficient for collection. In general, you can see high Ab concentrations in 7-8 tubes. Test 1 ul on pH paper. Read OD to know Ab concentrations. OD (Absorbance at 280 nm)/1.35 = X
技术资料暂无技术资料 索取技术资料
