- ￥58234
- Beckman Coulter贝克曼库尔特
- 美国
- A29174
- 2025年07月08日
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文献和实验concentration of 200nM. All procedures should be done on ice. (2) qRT-PCR conditions and instrument setup a. Open the Mx4000 program and set up. Define the reactions of cDNA templates as“Unknown” and H2O templates as “NTC” (No Template Control). b
Methods for use with the mTn-lacZ/LEU2-mutagenized library
to check digestion. This gel may also be used for Southern analysis (see below). The remainder is heated to 65o C for 25 minutes to inactivate the restriction enzyme, and 215 ul of H2O, 25 ul of 10X ligase buffer and 1 ul of ligase (400 units) are added
Methods for use with the mTn-3xHA/lacZ-mutagenized library
of such logarithmically-dividing cultures increases transformation efficiency. Cells are pelleted and washed once with 5 volumes of One Step buffer (0.2M LiAc, 40% PEG 4000, 100 mM beta-mercaptoethanol). This wash is especially important when culture volumes
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