- ￥200
- Beckman Coulter贝克曼库尔特
- 美国
- 804487
- 2025年07月10日
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
TOP10 chemically competent cells
and screw cap tubes before use. Preparing seed stocks Streak TOP10 cells on an SOB plate and grow for single colonies at 23°C room temperature works well Pick single colonies into 2 ml of SOB medium and shake overnight at 23
Drill 10 matching 8/32" T.P.I. holes in top and bottom chambers for use with 8/32" flat head screws. 4. Flow circuit assembly 1) Assemble the entire flow circuit first, then proceed with its sterilization. 2)
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