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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
°C 5. Sterile serological pipettes (5-mL, 10-mL) 6. Centrifuge 7. 15-mL centrifuge tubes 8. 60-mm and 100-mm tissue culture-treated dishes 9. 6-well tissue culture-treated plates 10. 25-gauge 1½-inch needle
Fastfilter Plasmid Midi Kit Spin Protocol
Column: 10. Transfer 4 ml of the clear lysate to the HiBind® DNA Midi column assembled in the 15 ml collection tube. The Midi column has a maximum capacity of 4.5 ml. Centrifuge at 3,000-5,000 x g for 3-5 min at room temperature to completely pass
Fastfilter Plasmid Maxi Kit Spin Protocol
Column into a 50 ml collection tube, provided. Add 5 ml of Buffer GPS to the column and Lit it sit at room temperature for 3-10 min. Spin in a swinging bucket rotor at 3,000-5,000 x g for 5 minutes at room temperature. Discard the eluate and assemble
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