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- Beckman Coulter贝克曼库尔特
- 美国
- 356252
- 2025年07月14日
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文献和实验Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage
from side to bottom. It will take approximately 6 minutes for the sample temperature to reach start temperature of 4 degrees C on the chart drive. Resuspend cell pellet with 10 ml of freezing media. Pipette 1.0 ml into each of 10 cryotubes on ice. DMSO is toxic to cells
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
, base, antifoam, inoculation. 1 each; drain valve steam, vessel drain port steam, transfer line condensate, sampling steam). 5. Check that all bolts on headplate are tightened. 6. Check water filter and change if necessary. (the filter
Method: Lymphoblastoid Cell Lines from Frozen Whole Blood
µl dimethyl sulfoxide (DMSO), or 10% of the volume of blood. Immediately begin freezing the whole blood in the cryomed freezing chamber until the chart drive printer reads -90 degrees C.
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