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文献和实验全基因组的比较基因组杂交技术介绍(Whole-Genome and&
Service Overview NimbleGen's CGH microarray service consists of the following steps: Customer and NimbleGen create custom array design, and NimbleGen manufactures the array. Customer supplies 1-3µg purified, undegraded sample
R&D Systems:如何提高MSC的扩增且不损失其分化潜能
。人MSCs (hMSCs ) 购于Cambr e x ( Lonz a ,Walkersville),先根据供应商的说明书培养,然后用StemXVivo人/小鼠骨髓间充质干细胞扩增培养基( 目录# C C M 0 0 4 ; R & DSystems,Minneapolis)培养。为了测试不同批次FBS的扩增潜能,每种F B S 以终浓度为1 0 % 或2 0 % 加入含有谷氨酰胺, 丙酮酸钠( I r v i n e S c i e n t i c , S a n t a A n a ) 的α
with the BioBrick) to allow for the fragments to be fused together in the In-Fusion reaction (see figure below). This method can be adapted to fuse more than two BioBricks together and four fragments have been successfully fused. This method can also be used
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