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文献和实验C oven (Horvitz lab: this is in the autoclave room). Plasticware (pipette tips, centrifuge tubes, 10 ml disposable pipettes) should be from an unopened package that is labelled RNase free and then kept in a special drawer after opening
秀丽线虫RNA提取--C. elegans RNA prep
with this enzyme. Glassware (but not the plastic caps!) stir bars, and spatulas for weighing reagents out should be baked overnight in a 180° C oven (Horvitz lab: this is in the autoclave room). Plasticware (pipette tips, centrifuge tubes, 10 ml disposable pipettes
- strains can be used. The commonly available strains, HB101 or DK1, the common BAC host, DH10B, and the common P1 host, NS3145, give good results. IMPORTANT! Make sure that your electrocompetent cells give 10.8, or greater, colonies per microgram
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