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文献和实验image of a whole mount from a 4 wk old virgin Protocol Spread tissue on glass slide Fix in Carnoy's fixative for 2 to 4 hours at r.t. Wash in 70 % EtOH for 15 min Change gradually to distilled water
Immunofluorescence- Whole Mount
in siliconized 1.6mL microfuge tubes. Remove PBST with pasteur pipette being careful to leave some residual fluid and embryos behind and undamaged. The same should be done prior to each wash below, unless otherwise stated. Wash embryos 1 X
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBR
Dechorionate directly on apple juice/agar plates with 50-60 chlorox; embryos will float to the surface after 2-3''. Collect embryos on a Millipore device with a nitex filter; rinse well with 0.1 Triton X-100 from a squirt bottle
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