USER MANUAL, HIGH PRESSURE DIF

Comprehensive High‐Throughput Arrays for Relative Methylation (CHARM)

to enable the examination of DNAm across the genome. In this unit, we describe comprehensive high?throughput arrays for relative methylation (CHARM), a highly sensitive and specific approach to measure DNA methylation across the genome. This method makes

Using ProSight PTM and Related Tools for Targeted Protein Identification and Characterization with High Mass Accuracy Tandem MS Data

, and the Sequence Gazer allow the identification and characterization of proteins from high mass accuracy tandem mass spectrometric data of intact proteins and large peptides. Input data consists of one or more neutral precursor ion masses and a set of neutral b/y

Obtaining Embryos: hCG Injection

to lay eggs at about 4 hours. The frogs are kept at room temperature throughout the priming and boosting periods. Attempts to shorten the period between boost and egg laying by increasing the temperature (as high as 30ºC) have been unsuccessful