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- Beckman Coulter贝克曼库尔特
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- 2025年07月11日
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文献和实验, single-stranded (ss) (denatured) DNA or RNA is bound to a filter support (e.g., nitrocellulose) and incubated with a radioactively labeled ss DNA or RNA fragment (“probe”) complementary to the nucleic acids of interest. In subsequent washing steps
Pre-hyb/hyb for Radioactive Probes
Pre-hyb/hyb for Radioactive Probes Stuff you need: 50X Denhardt's reagent: 1% Ficoll 1% Polyvinylpyrrolidone 1% Bovine Serum Albumin Prehyb/hyb solution: 5X SSC 50% formamide 100 µg/ml SS DNA (heat denatured at 95 deg C) 5X
Denaturing Gradient Gel Electrophoresis (DGGE)
with a radioactive probe, washed, and exposed to X-ray film for 1-5 days. Each lane is examined for fragments that have mobility shifts. Many polymorphisms are present in portions of a restriction fragment not in the first melting domain. Sequence differences
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