相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海博耀商贸有限公司
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Purification of GST fusion proteins in E.coli GST融合蛋白纯化,方法一
Quick spin,combine supernatant,spin and transfer supernatant twice to avoid any residual beads.total is 120μl now. Dialyze vs 50% glycerol/10mM Hepes,pH7.5/ 40mM KCl/ 1mM EDTA/ 1mM DTT/ 1mM PMSF in cold room for 2hr or o/n,store at -20℃ Proteins
Home-made Taq Polymerase Purification
) with 15ml of an overnight of Taq bugs. These volumes may be scaled down. 2) Grow to an OD600 of 0.6 (approx mid log) 3) Add IPTG to 0.5mM (0.119gm/litre), grow o/n but not for more than 16hrs. Day 2 N.B: All the following proceedures
Tris Cl (pH 8.0 37o C)) 10mM 1M 10µl 100µl MgCl2 5mM 1M 5µl 50µl NaCl 100mM 5M 20µl 200µl b-Me 1mM 1M 1µl 10µl H2 O mQ 64µl 640µl L. Conc. Stock
技术资料暂无技术资料 索取技术资料





