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- 文献和实验
- 技术资料
- 保存条件：
见瓶身
- 保质期：
/
- 英文名：
/
- 库存：
999
- 供应商：
麦飞生物
- CAS号：
56-84-8
- 规格：
500 g
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文献和实验buffer or in your preferred buffer. The high concentration of nucleic acid in the isolated nucleoli makes the lysed sample very viscous. The sample can be clarified by passing through a QIAshredder spin column (Qiagen Cat no: 79654). Nucleoli
Replication timing by density transfer
. It may too high when you first make it (up to 1.4010 or thereabouts); just add 10 mM Tris until the refractive index comes down. After the CsCl is in solution, the refractive index should be 1.4052. It's worth adding 90 microliters of dummy restriction enzyme mix
, and 2) fractionation over BlueSepharose, which binds Wnts with high selectivity. The purificationconsists of four chromatography steps: Blue Sepharose, immobilized metal affinity chromatography (IMAC), gel filtration,and heparin cation exchange
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