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文献和实验PURIFICATION OF PCR PRODUCTS WITH SEPHADEX
Sephadex from Amersham Pharmacia; G-50 DNA grade F: Catalog no: 17-0573-02 Polyfiltronics Unifilters (from Fisher): Whatman ?Polyfiltronics, Unifilters ?96 wells, Cat no: 7700 - 3304
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
and STA-PCR protocols. B112, B68, and B75 are BXH-2-derived leukemia samples. NC: healthy BXH-2 mice tail DNA used as a control. M: 100-bp DNA marker. The whitearrows indicate the differential bands. The size of DNA marker in base-pair is noted tothe left
Agarose Gel Electrophoresis for the Separation of DNA Fragments
the lid. Slowly and carefully load the DNA sample(s) into the gel (Fig. 3). An appropriate DNA size marker should always be loaded along with experimental samples. 6) Replace the lid to the gel box. The cathode (black leads) should be closer
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