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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见瓶身
- 保质期：
/
- 英文名：
/
- 库存：
999
- 供应商：
麦飞生物
- CAS号：
/
- 规格：
100 ml
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文献和实验Assemble the following components: •Cells,grown in 10 cm dishes•Room Temp.PBS•Cold PBS•Cold Sonication Buffer (1% SDS,10 mM EDTA,50 mM Tris-HCl pH 7.5)Prepare sonication buffer as follows: 10 ml of 10% SDS,2 ml of 500 mM EDTA,5 ml of 1 M Tris,pH
TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
Immediately pour the solution into a one liter bottle which has been packed in ice. This cools the solution quickly and prevents breakdown of the paraformaldehyde. Store at 4°C for up to two weeks. 15% Sucrose in PBS: 500ml sterile
TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
should be rinsed in saline or PBS and frozen in O.C.T. blocks in liquid nitrogen as outlined above. Although not optimal, it is also possible to use snap frozen material tissue without an embedding matrix. The fixation, sucrose, and O.C.T. steps are used primarily
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