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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见瓶身
- 保质期：
/
- 英文名：
/
- 库存：
999
- 供应商：
麦飞生物
- CAS号：
1609-47-8
- 规格：
100 g
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文献和实验RNase and DEPC Treatment: Fact or Laboratory Myth
DEPC by causing hydrolysis of diethylpyrocarbonate. CO2 and EtOH are released as reaction by-products. DEPC has a half-life of approximately 30 minutes in water, and at a DEPC concentration of 0.1%, solutions autoclaved for 15 minutes/liter
RNA purification using TRizol--TRIZOL法提取RNA
glass bottles. b.Add 0.01% (v/v) diethylpyrocarbonate (DEPC). c.Let stand overnight. d.Autoclave. Note: RNase free DEPC treated water is Biotecx brand (cat # BL-5611). Note: If using 0.5% SDS solution to resuspend the RNA. It must be prepared
Fast and reliable mini-prep RNA extraction from Neurospora crassa
and redissolve it in DEPC treated (diethyl polycarbonate) water - Store the RNA solution at -70°C Purity of the RNA preparations was assayed by spectrophotometric measurements. The A260 /A230 and A260 /A280 ratios were 2 or more, indicating the absence
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