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文献和实验Bacterial Media Solutions and Stocks
2 Shelf life of 3 months PP3 RNAase (10 mg/ml) RNAase stock solution (5 mg/ml) 10 SDS (100 ml) SOB Media SOC Media 3M sodium acetate (100 ml) TCM buffer
Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
,transformation of ligation mixes and color selectability procedures.Time required: 1.Transformation - 1 hour2.Growth - approximately 16 hours for visible coloniesSpecial Reagents: 1.Competent Cells2.SOB Media (needed for DH5-ALPHA cells only)3.SOC Media (needed
characteristics, depending on the media, the temperature and the strain of cells utilized. For bacteria (and some algae, fungi and other eukaryotic tissue culture) it is possible to measure the growth of cell populations by calculating cell number or mass
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