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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见瓶身
- 保质期:
/
- 英文名:
/
- 库存:
999
- 供应商:
麦飞生物
- CAS号:
/
- 规格:
500 ml
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文献和实验96-well RNA In Situ Hybridization Protocol
lDNase I (100U, RNase-free) 1ml Total VolumeProbe Resuspension Buffer 5.0ml formamide 0.5ml 10X TE pH 7.4 4.4ml ddH2 O 0.1ml 10% Tween 10ml total volume Hybridization Buffer 150ml ultra pure redistilled formamide 60ml 20X SSC 6ml 50
96-well RNA In Situ Hybridization Protocol
volume Hybridization Buffer 150ml ultra pure redistilled formamide 60ml 20X SSC 6ml 50X Denhardts 7.5ml 10mg/ml tRNA 7.5ml 10mg/ml ssDNA (BOIL before adding) 0.3ml 50 mg/ml Heparin 3.0ml 10% Tween ****** ddH2 O 270ml Total
Non-fluorescent protein/RNA double-labeling
to the bottom) and wash the embryos well with methanol. Transfer the embryos to a 0.5 ml eppendorf tube. 2. Wash the embryos with 3% hydrogen peroxide in methanol for 15 min. Rinse well in methanol. 3. Wash 3 X 30 minutes with PBTH (filtered 1 X PBS, 0.1% Tween-20
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