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- AMRESCO
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- VWRV1B1812
- 2025年07月12日
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- 文献和实验
- 技术资料
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见瓶身
- 保质期：
/
- 英文名：
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- 库存：
999
- 供应商：
麦飞生物
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- 规格：
25 ml
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文献和实验Agarose Gels for Single Stranded DNA
1. Prepare 50X TAE as: 242 g Tris Base 57.1 mL Glacial Acetic Acid 100 mL 500 mM EDTA, pH 8.0 600 mL ddH2O Mix. Bring volume to 1 L. Autoclave. 2. Mix the following: 0.40 g Agarose 4.00 mL 50X TBE (4X TAE final) 46.00 mL
Agarose Gel Electrophoresis of DNA
it set, put comb in (leave a small gap between gel surface and comb) and pour 100ml of ~1% LMP agarose. This method helps the low m.p agarose to set and provide a firm base for the handling of the gel. j) When loading DNA sample into the well of your gel
(Laemmli loading dye) 3X stock:1M Tris-Cl pH 6.8 2.4 ml20% SDS 3 mlGlycerol (100%) 3 mlB-mercaptoethanol 1.6 mlBromophenol blue 0.006g10 ml (store 4°C)10X Running buffer (also called Laemmli buffer):Tris base 30.3 gGlycine 144 gSDS 10 gmake to 1L with dH
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