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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
986
- 靶点:
β2-microglobulin
- 级别:
β2-microglobulin Antibody
- 目录编号:
β2-microglobulin Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
β2-microglobulin Antibody
- 抗体英文名:
Dazzle™ 594 anti-human β2-microglobulin Antibody-PE
- 抗体名:
β2-microglobulin Antibody
- 标记物:
PE
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
β2-microglobulin Antibody
- 亚型:
β2-microglobulin Antibody
- 形态:
β2-microglobulin Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
β2-microglobulin Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥3000.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥6500.0 |
Description :β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membraneanchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavychains of MHC class I molecules to form HLA class I antigen complex. In association with HLAclass I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2Mplays an essential role both in governing MHC class I molecules stability and in promotingantigen binding and presenting the antigen to CD3/TCR complex of CD8 T cells.
Verified Reactivity :Human
Reported Reactivity :African Green, Baboon, Cynomolgus, Rhesus, Pig
Antibody Type :Monoclona
Host Species :Mouse
Immunogen :Purified human β2-microglobulin
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Meissner TB, et al. 2010. Proc Natl Acad Sci USA. PubMed
2. Rizvi SM, et al. 2011. J. Immunol. 186:2309. PubMed
3. Meissner TB, et al. 2012. J Immunol. 188:4951. PubMed.
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择: 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照,是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型
非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型对照选择PE标记的小鼠的IgG2a。 流式抗体使用注意事项: 1. 建议实验细胞的数量和抗体的比例要适当。细胞过量或抗体过量都可能使实验结果受影响,因此需要优化试验条件。注:不同的流式技术对抗体的需求量有较大差异,例如传统流式细胞术(采用鞘液流系统)需要1×106个细胞为起始上样量,抗体用量为10 μl










