Control Normal Mouse IgG3 [B10

Analysis of Gene Expression in Hypothalamus in Obese and Normal Mice Using Differential Display

, a hormone made in fat that regulates hypothalamic function, was only recently identified (7 ). While NPY is a potent stimulator of eating behavior, the NPY knockout mouse (8 ) exhibits a normal feeding phenotype, indicating that other factors are involved

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

BAC Transgenes, DNA Purification, and Transgenic Mouse Production

expression occurs at physiological levels in the appropriate cell types while recapitulating normal developmental time frames. Genomic libraries prepared in bacterial artificial chromosomes (BACs) for the mouse and human genome sequencing projects are a ready