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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos
- 保存条件:
Store vial at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
WB, IHC-Fr, Flow, IP
- 抗体英文名:
MCAM Antibody [C264] (PE)
- 抗体名:
MCAM Antibody [C264] (PE)
- 规格:
0.1mg
MCAM 抗体 [C264] (PE),MCAM Antibody [C264] (PE),MCAM 抗体 [C264] (PE),MCAM Antibody [C264] (PE)
产品名称:MCAM 抗体 [C264] (PE)-MCAM Antibody [C264] (PE)
产品货号:PSI-99-305-0.1mg
产品规格:0.1mg
背景资料:Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm’s kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes.
保存建议:MCAM 抗体 [C264] (PE)-MCAM Antibody [C264] (PE)Store vial at 2-8℃
应用类型:WB, IHC-Fr, Flow, IP
点击:MCAM 抗体 [C264] (PE)-MCAM Antibody [C264] (PE)查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
PCR -------------------------------------------------------------------------------- Optimal Annealing Temperature: 54.0° (Max: 70.9°) -------------------------------------------------------------------- Position Length Tm [°C] GC [%] P.E
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