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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos
- 保存条件:
Store vial at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
IHC-Fr, Flow, IP
- 抗体英文名:
MCAM Antibody [C264]
- 抗体名:
MCAM Antibody [C264]
- 规格:
0.5mg
MCAM 抗体 [C264],MCAM Antibody [C264],MCAM 抗体 [C264],MCAM Antibody [C264]
产品名称:MCAM 抗体 [C264]-MCAM Antibody [C264]
产品货号:PSI-99-302-0.5mg
产品规格:0.5mg
背景资料:Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm’s kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes.
保存建议:MCAM 抗体 [C264]-MCAM Antibody [C264]Store vial at 2-8℃
应用类型:IHC-Fr, Flow, IP
点击:MCAM 抗体 [C264]-MCAM Antibody [C264]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验细胞学堂丨养不好RAW 264.7,是因为忽略了这几点,RAW264.7分化有哪些问题要注意
和培养器皿的材料也有关,有时RAW264.7细胞会出现太容易吹下的情况,连分化细胞都贴壁较松,此时更适宜用巴氏管吹打; 4. 培养时,无法保证100% 不分化,通过传代可以将分化比例控制在一定范围 5. RAW264.7细胞分裂活跃,记得每天看一看,周末也要抽点时间关心一下它哦~ 小知识 RAW 264.7细胞系的起源 加利福尼亚索尔克研究所的W. C. Raschke在雄性BAB/14小鼠腹腔注射A-MuLV(Abelson鼠科白血病病毒)诱发肿瘤的腹水中建立了RAW 264细胞
的既有鼠源成分,又有人源成分的抗体称为人源化抗体(humanized antibody)。人源化 抗体是通过基因工程制备的,所以又可称为基因工程抗体(genetically engineered antibody)。人源化抗体 的优越性主要表现在于其不会激起快速的抗球蛋白反应。由于Fc 段来源于人,其能更有效的与人体免疫 系统协同发挥作用。 嵌合抗体 由于90%的HAMA 是针对抗体C 区的,设计从分泌鼠McAb 的杂交瘤细胞基因组中分离功能性IgV 区基因,然后与人C 区基因按一定方式拼接
, or as often as necessary to decrease the background. Pellet cells. Save antibody supernatant, which is now diluted 1:5 from its initial concentration. These antibodies can be stored at 4o C for several weeks or at -70o C for longer periods. Solutions
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