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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CE mouse spleen enriched for NK-1+ cells and bone marrow cells
- 保存条件:
Store vial at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
IHC-Fr, Flow, Blocking, IP
- 抗体英文名:
NK1.1 Antibody [PK136] (PE-Cy7)
- 抗体名:
NK1.1 Antibody [PK136] (PE-Cy7)
- 规格:
0.1mg
NK1.1 抗体 [PK136] (PE-Cy7),NK1.1 Antibody [PK136] (PE-Cy7),NK1.1 抗体 [PK136] (PE-Cy7),NK1.1 Antibody [PK136] (PE-Cy7)
产品名称:NK1.1 抗体 [PK136] (PE-Cy7)-NK1.1 Antibody [PK136] (PE-Cy7)
产品货号:PSI-98-957-0.1mg
产品规格:0.1mg
背景资料:NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL/6, NZB, and CE). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.
保存建议:NK1.1 抗体 [PK136] (PE-Cy7)-NK1.1 Antibody [PK136] (PE-Cy7)Store vial at 2-8℃
应用类型:IHC-Fr, Flow, Blocking, IP
点击:NK1.1 抗体 [PK136] (PE-Cy7)-NK1.1 Antibody [PK136] (PE-Cy7)查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA
管中。必要时可将界面放在白色背景前以便看清。用RPMI-10完全培养基洗2遍(方法同步骤6)。台盼蓝染色计数活细胞,备用。 12. 为检验细胞纯度,可加入终浓度为10mg/ml的抗CD3-FITC和PK136-PE(抗NK1.1)抗体,进行流式分析。 附录: HBSS/10%(V/V)FBS和HBSS/3%(V/V)FBS:56℃ 45min热灭活FBS,过滤。取HBSS,加入不同量的FBS,分别配置含10%和3%(V/V)FBS的HBSS溶液。在10%FBS的HBSS溶液中加入酚红
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