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- ProSci
- 美国
- PSI-98-637-0.1mg
- 2025年07月10日
- E
- 大鼠
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Fc Fragment of IgG Low Affinity IIIa Receptor (FCGR3A) Antibody (PE / DL594)/Fc Fragment of IgG Low Affinity IIIa Receptor (FCGR3A) Antibody (PE / DL594)/Fc Fragment of IgG Low Affinity IIIa Receptor (FCGR3A) Antibody (PE / DL594)
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peritoneal macrophages from B6D2 hybrid mice
- 保存条件：
Store vial at 2-8℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
大鼠
- 应用范围：
E
- 抗体英文名：
CD11b Antibody [3A33] (PE-Cy5)
- 抗体名：
CD11b Antibody [3A33] (PE-Cy5)
- 规格：
0.1mg
CD11b 抗体 [3A33] (PE-Cy5)，CD11b Antibody [3A33] (PE-Cy5)，CD11b 抗体 [3A33] (PE-Cy5),CD11b Antibody [3A33] (PE-Cy5)
产品名称：CD11b 抗体 [3A33] (PE-Cy5)-CD11b Antibody [3A33] (PE-Cy5)
产品货号：PSI-98-637-0.1mg
产品规格：0.1mg
背景资料：CD11b (integrin alphaM subunit) combines with CD18 (integrin beta2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.
保存建议：CD11b 抗体 [3A33] (PE-Cy5)-CD11b Antibody [3A33] (PE-Cy5)Store vial at 2-8℃
应用类型：E
点击：CD11b 抗体 [3A33] (PE-Cy5)-CD11b Antibody [3A33] (PE-Cy5)查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA
分泌。 随后进行表面染色：先以Fc阻断剂（2.4G2克隆，5 μg/mL）处理10分钟，再于4°C避光孵育15分钟，使用含1% BSA和0.1% NaN₃的DPBS缓冲液配制抗体，常用组合包括CD90.2、CD4、CD8、CD25、CD11b和CD45.2等标记。 固定步骤采用2%甲醛（FA，由含<3%甲醇的4% Roti-Histofix稀释），室温孵育40–60分钟。破膜与胞内染色使用0.1%皂苷缓冲液或1×Perm缓冲液，加入抗FoxP3、T-bet、RORγt、IL-17A或IFN-γ等抗体，4°C
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