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- ProSci
- 美国
- PSI-98-578-0.1mg
- 2025年07月14日
- E
- 大鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
IL-4 producing Th2 cell lines including D10
- 保存条件：
Store vial at 2-8℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
大鼠
- 应用范围：
E
- 抗体英文名：
CD3e Antibody [C363.29B] (PE)
- 抗体名：
CD3e Antibody [C363.29B] (PE)
- 规格：
0.1mg
CD3e 抗体 [C363.29B] (PE)，CD3e Antibody [C363.29B] (PE)，CD3e 抗体 [C363.29B] (PE),CD3e Antibody [C363.29B] (PE)
产品名称：CD3e 抗体 [C363.29B] (PE)-CD3e Antibody [C363.29B] (PE)
产品货号：PSI-98-578-0.1mg
产品规格：0.1mg
背景资料：CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25 kDa epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.2B9 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The monoclonal antibody is cytolytic, easily used for cell surface staining, and a good immunoprecipitating antibody.
保存建议：CD3e 抗体 [C363.29B] (PE)-CD3e Antibody [C363.29B] (PE)Store vial at 2-8℃
应用类型：E
点击：CD3e 抗体 [C363.29B] (PE)-CD3e Antibody [C363.29B] (PE)查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验A/B/C Random Amplification Protocol
the Round B PCR program and place the samples on the machine when it reaches 94˚C. Part B -- PCR machine parameters: (94˚C 3’) x1 (94˚C 30”, 40˚C 30”, 50˚C 30”, 72˚C 1’) x35 cycles (4ºC hold) x1 3. To check if the PCR reaction went
Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
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