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- ProSci
- 美国
- PSI-76-132-0.1mg
- 2025年07月13日
- Flow
- 大鼠
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
protected from prolonged exposure to light
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
大鼠
- 应用范围：
Flow
- 抗体英文名：
CD11b Antibody [M1/70] (PE-Cy7)
- 抗体名：
CD11b Antibody [M1/70] (PE-Cy7)
- 规格：
0.1mg
CD11b 抗体 [M1/70] (PE-Cy7)，CD11b Antibody [M1/70] (PE-Cy7)，CD11b 抗体 [M1/70] (PE-Cy7),CD11b Antibody [M1/70] (PE-Cy7)
产品名称：CD11b 抗体 [M1/70] (PE-Cy7)-CD11b Antibody [M1/70] (PE-Cy7)
产品货号：PSI-76-132-0.1mg
产品规格：0.1mg
背景资料：The M1/70 monoclonal antibody specifically reacts with the 170 kDa alphaM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18). Mac-1 binds to C3bi, CD54 (ICAM-1), and fibrinogen, and it is expressed by granulocytes, macrophages, NK cells, myeloid-derived dendritic cells, microglia, activated lymphocytes, and mouse B-1 cells. The expression is up-regulated on activated neutrophils at the same time that L-selectin is shed from the cell surface. The M1/70 antibody is used for the detection of monocytes, granulocytes, and a subset of natural killer cells in human peripheral blood.M1/70 blocks C3bi binding and cell adherence, but not cell-mediated lysis and it cross-reacts with human CD11b.
保存建议：CD11b 抗体 [M1/70] (PE-Cy7)-CD11b Antibody [M1/70] (PE-Cy7) protected from prolonged exposure to light
应用类型：Flow
点击：CD11b 抗体 [M1/70] (PE-Cy7)-CD11b Antibody [M1/70] (PE-Cy7)查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA
式染色带来的影响我们才能更好的做好流式实验。固定对荧光染料的影响是什么？主要表现在固定剂对串联染料（tandem-dye，如 APC/Cy7、PE/Cy7 等）的影响：一个是固定剂有可能造成串联染料的断裂，比如 APC-Cy7 和 4%PFA 接触时间超过 4 个小时染料就有可能断裂，所以如果细胞直接重悬在固定液里面建议尽快上机，如果要放到第二天上机，需离心去除固定液后重悬在流式染色液或者是 PBS 里面，当然有一些新的串联荧光素可以有效减少这种情况，比如 BioLegend 的 APC/Fire750TM
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