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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant CD20 protein was used as the immunogen for this CD20 antibody. The epitope is known to be extracellular.
- 保存条件:
Aliquot and Store at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
Flow, IF, IHC
- 抗体英文名:
CD20 Antibody [CDLA20-1]
- 抗体名:
CD20 Antibody [CDLA20-1]
- 规格:
100ug
CD20 抗体 [CDLA20-1],CD20 Antibody [CDLA20-1],CD20 抗体 [CDLA20-1],CD20 Antibody [CDLA20-1]
产品名称:CD20 抗体 [CDLA20-1]-CD20 Antibody [CDLA20-1]
产品货号:PSI-33-937-100ug
产品规格:100ug
背景资料:CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
保存建议:CD20 抗体 [CDLA20-1]-CD20 Antibody [CDLA20-1]Aliquot and Store at 2-8℃
应用类型:Flow, IF, IHC
点击:CD20 抗体 [CDLA20-1]-CD20 Antibody [CDLA20-1]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验CD20 分子 CD20 常用单克隆抗体或代号: B1,Leu16 主要表达细胞: B,FDC [B] 分子质量(kDa)和结构: p33(非糖基化穿膜磷蛋白) 功 能: Ca2+通道,调节B细胞活化和增殖 CD20 Aka L26 33kd phosphoprotein with 3 hydrophobic regions that traverse the cell
【摘要】目的探讨嵌合抗CD20抗体Fab’片段的抗肿瘤机制。方法应用MTT法检测Fab’片段对Raji细胞生长的抑制作用,用Annexin V-FITC和PI测定Fab’片段对Raji细胞凋亡的诱导作用,用RT-PCR和Western blot检测Raji细胞中bcl-2表达的变化。结果嵌合抗CD20Fab’片段对Raji细胞具有明显的抑制作用IC50值为24.2 µg/ml,并呈剂量依赖性;嵌合抗CD20Fab’片段能诱导Raji细胞的凋亡,并降低Raji细胞中bcl-2基因及其蛋白的表达
【摘要】为了研究嵌合抗CD20基因工程抗体Fab的抗肿瘤活性及其抗肿瘤机制,本研究利用3H掺入法测定嵌合抗CD20 Fab对Raji细胞生长的影响,结果显示嵌合抗CD20 Fab对Raji细胞的生长具有抑制作用;利用流式细胞仪测定嵌合抗CD20 Fab诱导Raji细胞凋亡作用,结果显示嵌合抗CD20 Fab可诱导Raji细胞凋亡作用。这些实验结果证明嵌合抗CD20 Fab通过诱导Raji细胞凋亡的机制抑制Raji细胞生长。【关键词】B淋巴细胞瘤嵌合FabCD20 凋亡【前言】CD20
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