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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified human S100A protein was used as the immunogen for this S100 antibody.
- 保存条件:
Aliquot and Store at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
IF, IHC-p
- 抗体英文名:
S100A Antibody [S1-61]
- 抗体名:
S100A Antibody [S1-61]
- 规格:
100ug
S100A 抗体 [S1-61],S100A Antibody [S1-61],S100A 抗体 [S1-61],S100A Antibody [S1-61]
产品名称:S100A 抗体 [S1-61]-S100A Antibody [S1-61]
产品货号:PSI-33-750-100ug
产品规格:100ug
背景资料:S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S100 protein. Despite the fact that S100 protein is an ubiquitous substance, its demonstration is of great value in the identification of several neoplasms, particularly melanomas and their metastases.
保存建议:S100A 抗体 [S1-61]-S100A Antibody [S1-61]Aliquot and Store at 2-8℃
应用类型:IF, IHC-p
点击:S100A 抗体 [S1-61]-S100A Antibody [S1-61]查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验(or 2-3h @ RT). 8. Transform 100ml competent cells with 10ml ligation reaction; freeze remainder. EB 66mM Tris-HCl, pH 8 0.66mM MgCl2 10X Klenow Mix 20mM Tris-HCl, pH 8100mM MgCl2 S1B 40mM KOAc 300mM NaCl 1.3mM ZnS04 7% Glycerol Ligation Mix 50mM Tris
digested plasmid (cut 5 µg of plasmid in volume of 20 µl) 51 µl labeled oligo 10 µl 10X Taq Buffer 6 µl 25 mM MgCl2 10 µl DMSO 2 µl 10 mM dNTP's 1 µl Taq polymerase 14 cycles of PCR Run on 6-8% polyacrylamide/urea gel Cut out probe and elute S1
在植物受粉中支配花粉和花柱性质的基因。它决定着可育性和不育的性质。例如樱桃具有S1 、 S2 、 S3 、 S4 等 S基因,其具有 S1 S2 基因的花粉,即使着落于 S1 或 S2 基因型的花柱上,也不能在此花柱上发芽,而产生同一基因的自体不育。与此相反,在具有与花柱不同的 S基因 S3 或 S4 的花粉,便可在花柱上发芽伸长,并受精结果,产生具有 S1 、 S2 、 S3 、 S1 S4 、 S2 S4 等基因型的接合体而结出种子来。认为这种情况
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