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- ProSci
- 美国
- PSI-33-900-100ug
- 2025年07月15日
- Flow, IF, WB, IHC-P
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
- 保存条件：
Aliquot and Store at 2-8℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
小鼠
- 应用范围：
Flow, IF, WB, IHC-P
- 抗体英文名：
p27Kip1 Antibody [KIP27-1]
- 抗体名：
p27Kip1 Antibody [KIP27-1]
- 规格：
100ug
p27Kip1 抗体 [KIP27-1]，p27Kip1 Antibody [KIP27-1]，p27Kip1 抗体 [KIP27-1],p27Kip1 Antibody [KIP27-1]
产品名称：p27Kip1 抗体 [KIP27-1]-p27Kip1 Antibody [KIP27-1]
产品货号：PSI-33-900-100ug
产品规格：100ug
背景资料：This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene.
保存建议：p27Kip1 抗体 [KIP27-1]-p27Kip1 Antibody [KIP27-1]Aliquot and Store at 2-8℃
应用类型：Flow, IF, WB, IHC-P
点击：p27Kip1 抗体 [KIP27-1]-p27Kip1 Antibody [KIP27-1]查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
Mouse p27 PCR Using Gitschier Buffer
Mutant allele: Neo-1 primer (CCTTCTATCGCCTTCTTG), plus mgK-3 primer (TGGAACCCTGTGCCATCTCTAT) produce a 0.5kB PCR product. Wildtype allele: mgK-3 (above) plus mcK-5 (GAGCAGACGCCCAAGAAGC) produce ~1kB product. Stock Solutions
-induced responses, including a down-regulation of the cyclin-dependent kinase (Cdk2) inhibitors p21CIP1 and p27KIP1 , while attachment to P-L-L did not. We conclude that engagement of the FN-receptor integrin α5β1 induces integrin signaling regulating
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