CDK7 & CCNH & MNAT1 Protein, H

蛋白长度：A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Ala2-Phe346 & Tyr2-Leu323 & Asp2-Ser309

标签类型：N-His

分子量：The recombinant heterotrimer of human CDK7/CCNH/MNAT1 comprises 1032 (364 + 341 + 327) amino acids and has a calculated molecular mass of 118.8 (41.2 + 39.7 + 37.9) kDa. The apparent molecular mass of rh CDK7/CCNH/MNAT1 heterotrimer is approximately 25,38 & 44 kDa respectively in SDS-PAGE under reducing conditions.

活性：The specific activity is >5 nmol/min/mg using MBP as substrate.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：cyclin-dependent kinase 7

通用名：CDK7 & CCNH & MNAT1

蛋白序参考：P50613&P51946&P51948

总结：

形式：Liquid

纯度：> 85 % as determined by SDS-PAGE.

储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Supplied as sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

别名：CAK1 Protein, Human; CDKN7 Protein, Human; HCAK Protein, Human; MO15 Protein, Human; p39MO15 Protein, Human; STK1 Protein, Human