CD70 Protein, Human, Recombina

蛋白长度：A DNA sequence encoding the human CD70 (NP_001243.1) (Gln39-Pro193) was expressed with a N-terminal Fc region of human IgG1 tagged AVI tag at the N-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Gln39-Pro193

标签类型：N-hFc & AVI

分子量：The recombinant human CD70 consists of 408 amino acids and predicts a molecular mass of 45.7 kDa.

活性：Immobilized Human CD27 (His & hFc Tag)(Cat:10039-H03H) at 2 μg/mL (100 μL/well) can bind Human CD70 (ECD, hFc & AVI Tag), Biotinylated(Cat:PH50448M3), the ECis 8-68ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：CD70 molecule

通用名：CD70

蛋白序参考：NP_001243.1

总结：CD70, a member of the tumor necrosis factor superfamily, is restricted to activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor, CD27, is important in priming, effector functions, differentiation and memory formation of T-cells as well as plasma and memory B-cell generation. Tight control of CD70 expression is required to prevent lethal immunodeficiency. By selective transcription, CD70 is largely confined to activated lymphocytes and dendritic cells (DC). As a type II transmembrane receptor, CD70 is normally expressed on a subset of B, T and NK cells, where it plays a costimulatory role in immune cell activation. Immunohistochemical analysis of CD70 expression in multiple carcinoma types. The restricted expression pattern of CD70 in normal tissues and its widespread expression in various malignancies makes it an attractive target for antibody-based therapeutics. Investigations to exploit CD70 as a cancer target have lead to the identification of potential antibody-based clinical candidates.

形式：Lyophilized

纯度：> 80 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：CD27L Protein, Human; CD27LG Protein, Human; TNFSF7 Protein, Human