- ￥2500 - 6500
- 齐源
- 368503
- 国产/进口
- 2025年07月08日
- FC
- 小鼠
- 人
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD45
- 级别：
CD45 Antibody
- 目录编号：
CD45 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
CD45 Antibody
- 抗体英文名：
Cyanine5.5 anti-human CD45 Antibody/PerCP
- 抗体名：
CD45 Antibody
- 标记物：
PerCP
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
CD45 Antibody
- 亚型：
CD45 Antibody
- 形态：
CD45 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
CD45 Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥2500.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥6500.0 |
Description ：CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocytecommon antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasmamembrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signalingmolecule that regulates a variety of cellular processes including cell growth, differentiation, cellcycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigenreceptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, andother Src family kinases. CD45 non-covalently associates with lymphocyte phosphataseassociated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bindgalectin-1 and to be associated with several other cell surface antigens including CD1, CD2,CD3, and CD4.
Verified Reactivity ：Human
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Human PBMC
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5under optimal conditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
2. Csiba A, et al. 1984. Br. J. Cancer 50:699.
3. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
4. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations ：
1. Zaman R, et al. 2021. Immunity. :. PubMed
2. Fujino S, et al. 2021. Mol Cancer Res. 19:812. PubMed
3. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
4. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
5. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
6. Slingsby MHL, et al. 2021. Haematologica. Online ahead of print. PubMed
7. Hu G, et al. 2022. Cell Death Dis. 13:640. PubMed
8. Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
9. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
10. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
11. Ramond C et al. 2017. eLife. 6 pii: e27564. PubMed
12. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
大部分老师做流式实验时，会用到空白对照、单阳对照、同型对照、生物学对照等，用到 FMO 对照的比较少。尽管如此，FMO 对照在设置圈门时仍具有重要的参考价值。 一方面，FMO 对照有助于精确地设置阴阳性群体界限。我们在做多色实验时，可能会出现某些通道信号难以区分的情况，主要是因为其他某个荧光对该通道的干扰很大。FMO 对照（Fluorescence Minus One，荧光扣除一）减少了其中一个通道的荧光抗体，能够帮助衡量其它通道的荧光漏溢情况。 如下图所示，PerCP/Cyanine
69、CD38 等活化指标，及 IFN-γ、IL-4、IL17A 等细胞因子，则需要设置同型对照，实现阴阳性细胞群的良好区分。 CD4 CD25 （2）在某些特殊情况下，如单核细胞被 PE/Cyanine 7、APC/Cyanine 7 等串联染料标记的抗体染色时，使用同型对照抗体是十分有必要的。单核细胞一般倾向于与 Cyanine7 等染料非特异性结合，使用同型对照抗体有助于获得理想结果。 （3）不清楚抗原表达情况的时候，一定要使用同型对照来界定阴性细胞群。 （4）胞内染色时
Fluorochrome Absorption and Emission Spectra
. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on stream-in-air flow cytometers. PerCP-Cy5.5 is a tandem conjugate system than combines PerCP with a cyanine dye (Cy5.5™) and has an absorption maximum
