DiI碘化物

Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling

, consisting of specific neural cell types that develop in defined time periods. In this chapter we describe a method that labels entire NB lineages in the embryonic brain. Clonal DiI labeling allows us to follow the development of a NB lineage starting

碘化物缓冲液提取噬菌体单链DNA

碘化物缓冲液： 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 4 M NaCl。室温避光贮存。不知道这里面哪个是需要避光的？我怎么没有提出DNA来，不知道是为什么？用的是NEB手册上写的方法。1. 按上述方法进行噬菌斑扩增，在第一步离心后，将500 µl含噬菌体上清转入一新鲜离心管。2. 加200 µl PEG/NaCl，颠倒混匀，室温放置10 min。3. 离心10 min，弃上清液。4. 短暂离心，小心吸去残余上清。5. 沉淀物彻底重悬于100 µl碘化物缓冲液中

Whole Body MRI and Fluorescent Microscopy for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following

Methods to monitor transplanted stem cells in vivo are of great importance for potential therapeutic applications. Of particular interest are methods allowing noninvasive detection of stem cells throughout the body. Magnetic resonance imaging