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文献和实验Isolation of micro-RNA (miRNA)
Adapted from the Instruction manual of Stratagene''s Micro RNA Isolation Kit (Catalog #200344) Overview There are two main methods for isolating RNA: phenol-based extraction and silica matrix or glass fiber filter (GFF
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
-through. Then with the collection tube empty, centrifuge the spin cartridge at 10,000 x g for 2 min at room temperature to completely dry the HiBind® matrix. 11. Transfer the column to a clean 1.5 ml micro centrifuge tube (not supplied with kit) and elute the RNA with 30-50
Extraction of RNA for Bacteria
一、实验原理 The E.Z.N.A. HP Total RNA Kit uses the reversible binding properties of HiBind matrix, a new silica-based material. This is combined with the speed of mini-column spin technology. A specifically formulated high salt buffer
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