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文献和实验Carbonate Solution For Western Blot
CARBONATE WESTERN TRANSFER SOLUTIONSDunn, Anal. Biochem. 1986: 157 1.5L 1.0L 10mM NaHCO3 1.26g chill to 4oC
b-galactosidase Solution Assay
Solutions CPRG Assay Buffer 50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.5 1 mM MgCl2 0.1 ml 1M MgCl2 up to 100 ml with Q store at room temperature 1M KPO4 pH 7.5 250 ml 1M KH2PO4 (monobasic) 68.05 g up to 500 ml 100 ml 1M K2HPO4 (dibasic) 87
Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
HCl. 5. Vortex. The DNA is now in a 20 mM Tris/0.1 mM EDTA at pH 8.1 with about 12.5 mM Na++ ions. That's pretty close to the 10 mM Tris/ 0.1 mM EDTA / pH 8 solution used to store DNA. 6. Use 1 µl for PCR 7. (May need to microfuge for 6 minutes
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