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- 齐源
- 320207
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- 2025年07月16日
- ICFC
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
FOXP3
- 级别：
FOXP3 Antibody
- 目录编号：
FOXP3 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
FOXP3 Antibody
- 抗体英文名：
anti-human FOXP3 Antibody-PE
- 抗体名：
FOXP3 Antibody
- 标记物：
PE
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
FOXP3 Antibody
- 亚型：
FOXP3 Antibody
- 形态：
FOXP3 Antibody
- 应用范围：
ICFC
- 保存条件：
2-8℃
- 浓度：
FOXP3 Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥3000.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥6200.0 |
Description ：FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin,JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of Tregulatory cells than most cell surface markers (such as CD4 and CD25). Transducedexpression of FOXP3 in CD4 /CD25 cells has been shown to induce GITR, CD103, andCTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunityallergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice.Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggestingthat this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse,two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical fulllength sequence; the other (FOXP3 δ2) lacking exon 2. The 259D antibody recognizes humanFOXP3 epitope in the region of amino acids 105-235.
Verified Reactivity ：Human
Reported Reactivity ：Cynomolgus, Rhesus, Baboon, Chimpanzee
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Full-length FOXP3 protein
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography, and conjugated with PE under optimalconditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：ICFC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by intracellular flow cytometry using our TrueNuclear™ Transcription Factor Staining Protocol. Please check your vial or your CoA to find thesuggested use of this reagent per million cells in 100 µl staining volume. It is recommended that thereagent be titrated for optimal performance for each application.
Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
2. Yang ZZ, et al. 2006. Blood 107:3639. PubMed
3. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. PubMed
4. Groh V, et al. 2006. Nature Immunology 7:755. PubMed
5. Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656.PubMEd
6. Long SA, et al. 2008. J Autoimmun. 30:293. PubMed
7. Gong G, et al. 2009. Blood 113:837. PubMed
8. Long SA, et al. 2009. Eur J. Immunol. 39:612. PubMed
9. Long SA, et al. 2010. Diabetes. 59:407. PubMed
10. Ferraro A, et al. 2014. PNAS. 111:1111. PubMed
11. Vudattu NK, et al. 2014. J Immunol. 193:587. PubMed
12. Dupont G, et al. 2014. Cytokine. 69:146. PubMed
Product Citations：
1. Ariafar A, et al. 2020. Heliyon. 6:e05556. PubMed
2. Krebs FK, et al. 2021. Cancer Med. . PubMed
3. Zimmer N, et al. 2020. Cancers (Basel). 12:00. PubMed
4. Eberhardt K, et al. 2015. Clin Infect Dis. 61: 1615 - 1623. PubMed
5. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
6. Burbano YCB, et al. 2019. Rev Soc Bras Med Trop. 52:e20190101. PubMed
7. Park K, et al. 2008. Cancer Res. 68:8400. PubMed
8. Weiss E, et al. 2011. J Immunol. 187:1684. PubMed
9. , et al. 2021. Eur J Immunol. 51:2708. PubMed
10. Popmihajlov Z, Smith K 2008. PLoS One. 1.222916667. PubMed
11. Voo K, et al. 2009. Proc Natl Acad Sci U S A. 106:4793. PubMed
12. Cheng J, et al. 2022. iScience. 25:103588. PubMed
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sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗，这种抗体有单抗的吧？ 用于FCM： 1：20-100，这是说用于流式的稀释倍数，是不是稀释倍数越小越好呢？ 同型对照抗体是不是必须要做的？如何选择阴型同型对照抗体呢！ 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助！ freecell 抗体的稀释倍数是需要优化
人Foxp3 ( Foxp3 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Foxp3 单抗包被于酶标板上，标准品和样品中的 Foxp3与单抗结合，加入生物素化的抗人 Foxp3 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，Foxp3 浓度与 OD 值
