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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD80
- 级别：
CD80 Antibody
- 目录编号：
CD80 Antibody
- 克隆性：
单克隆
- 抗原来源：
仓鼠
- 保质期：
CD80 Antibody
- 抗体英文名：
Brilliant Violet 711™ anti-mouse CD80 Antibody
- 抗体名：
CD80 Antibody
- 标记物：
CD80 Antibody
- 宿主：
仓鼠
- 适应物种：
小鼠
- 免疫原：
CD80 Antibody
- 亚型：
CD80 Antibody
- 形态：
CD80 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.2 mg/ml
- 规格：
50 µg
Description ：CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is alsoknown as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells andmonocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation ofCD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendriticcells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cellactivation, proliferation, and cytokine production. CD80 appears to be expressed later in theimmune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliveran inhibitory signal to T cells.
Verified Reactivity ：Mouse
Reported Reactivity ：Dog
Antibody Type ：Monoclonal
Host Species ：Armenian Hamster
Immunogen ：CHO cell line transfected with mouse B7 (CD80)
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation ：The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™under optimal conditions.
Concentration ：0.2 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm isrecommended for detection, although filter optimization may be required depending on otherfluorophores used. Be sure to verify that your cytometer configuration and software setup areappropriate for detecting this channel. Refer to your instrument manual or manufacturer forsupport. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.
Excitation Laser ：Violet Laser (405 nm)
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文献和实验1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
5. Ma XT, et al. 2006. Cancer Res. 66:1169.
6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
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