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文献和实验In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase
The native T7 DNA polymerase is a fast and highly processive enzyme that can be used for in situ detection of apoptosis and various types of DNA breaks. The technique is quick and simple, and was shown to label earlier stages
DNA Sequencing Using Sequenase Version 2.0 T7 DNA Polymerase
The dideoxy chain-termination method of DNA sequence analysis involves the synthesis of a DNA strand by enzymatic extension from a specific primer using a DNA polymerase (1 ). Several different enzymes are available for this purpose, each having
mRNA Amplification with T7 RNA Polymerase
on ice. Second-strand cDNA synthesis Set PCR machine to hold at 16ºC. On ice prepare 2nd Strand Master Mix (per sample): 63 µL Nuclease-free H2O 10 µL 10x 2nd strand buffer 4 µL dNTP mix 2 µL DNA polymerase 1 µL
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