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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
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5,000 U @ 50 U/ul
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文献和实验mRNA Amplification with T7 RNA Polymerase
on ice. Second-strand cDNA synthesis Set PCR machine to hold at 16ºC. On ice prepare 2nd Strand Master Mix (per sample): 63 µL Nuclease-free H2O 10 µL 10x 2nd strand buffer 4 µL dNTP mix 2 µL DNA polymerase 1 µL
Dideoxy Sequencing Reactions Using T7 Polymerase
The chain termination DNA sequencing procedure introduced by Sanger and coworkers in 1977 (1 ) meant a revolutionary achievement in fast, simple, and accurate deciphering of DNA fragments. Long stretches of this nucleic acid
Oocyte Expression With Injection of Purified T7 RNA Polymerase
of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
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