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- 齐源
- 320105
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- 2025年07月10日
- ICFC
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
FOXP3
- 级别：
FOXP3 Antibody
- 目录编号：
FOXP3 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
FOXP3 Antibody
- 抗体英文名：
anti-human FOXP3 Antibody-FITC
- 抗体名：
FOXP3 Antibody
- 标记物：
FITC
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
FOXP3 Antibody
- 亚型：
FOXP3 Antibody
- 形态：
FOXP3 Antibody
- 应用范围：
ICFC
- 保存条件：
2-8℃
- 浓度：
FOXP3 Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥2700.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥5600.0 |
Description ：FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin,JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of Tregulatory cells than most cell surface markers (such as CD4 and CD25). Transducedexpression of FOXP3 in CD4 /CD25 cells has been shown to induce GITR, CD103, andCTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunityallergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice.Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggestingthat this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse,two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical fulllength sequence; the other (FOXP3 δ2) lacking exon 2. The 206D antibody recognizes humanFOXP3 epitope in the region of amino acids 105-235.
Verified Reactivity ：Human
Reported Reactivity ：Baboon, Cynomolgus, Rhesus, Pigtailed Macaque
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Full-length FOXP3 protein
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography, and conjugated with FITC under optimalconditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protectedfrom prolonged exposure to light. Do not freeze.
Application ：ICFC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by intracellular flow cytometry using our TrueNuclear™ Transcription Factor Staining Protocol. Please check your vial or your CoA to find thesuggested use of this reagent per million cells in 100 µl staining volume. It is recommended that thereagent be titrated for optimal performance for each application.
Excitation Laser ：Blue Laser (488 nm)
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文献和实验1. Roncador G, et al. 2005. Eur. J. Immunol. 35:1681.(IHC)
2. Yang ZZ, et al. 2006. Blood 107:3639.
3. Liu W, et al. 2006. J. Exp. Med. 203:1701.PubMed
4. Bollyky PL, et al. 2007. J. Immunol. 179:744.
5. Bell MP, et al. 2007. J. Immunol. 179:1893.
6. Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656. PubMed
7. Gao Q,et al.2007.J Clin Oncol.25:2586.(IHC) PubMed
8. Pillai V,et al. 2008. Blood 111:463. PubMed
9. Zheng Y, et al. 2008. J. Immunol. 181:1683. PubMed
10. Zonios DI, et al. 2008.Blood112:287. PubMed
11. Kavanagh B, et al. 2008. Blood. PubMed
12. Nevala WK, et al. 2009. Clin Cancer Res. 15:1931. PubMed
13. Grant J, et al. 2009. Cytometry B Clin Cytom. 76:69. PubMed
14. Nigam P, et al. 2010. J. Immunol. 184:1690. PubMed
15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (ICFC) PubMed
16. Hartigan-O'Connor DJ,et al.2007.J Exp Med.204:2679. PubMed
17. Raghaven S, et al. 2009. Ann Rheum Dis. 68:1908. PubMed
18. Hodi FS, et al. 2014. Cancer Immunol Res. 2:632.(IHC) PubMed
19. Sziros E, et al. 2015. Clin Cancer Res. 21:2840.(IHC) PubMed
Product Citations：
1. Johnson LE, et al. 2017. J Immunother Cancer. 5:56. PubMed
2. Rasouli J, et al. 2015. J Immunol. 194:5085. PubMed
3. Servais S, et al. 2015. PLoS One. 10:130026. PubMed
4. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
5. Alenazy MF, et al. 2021. Sci Rep. 11:5629. PubMed
6. Dayoub O, et al. 2017. Sci Rep. 10.1038/s41598-017-09933-4. PubMed
7. Mason R, et al. 2008. Virology. 381:11. PubMed
8. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
9. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
10. Rochman Y, et al. 2015. PLoS One. 10:122198. PubMed
当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成； 方法与步骤： 根据Marshall氏法高效价的抗人球蛋白兔免疫血清，分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg，缓冲液为总量的10%； 2. 将以上溶液降温至4℃，按蛋白：荧光素=50—80mg：1mg的比例加入异硫氰酸荧光素，在0—4℃下电磁搅拌12—14h； 3.用半饱和硫酸铵将标记球蛋白
抗体二抗上连接有荧光染料。直接标记染色背景低，实验程序少尽量减少实验工序和过程，以保证实验的真实和准确性。因此在条件允许的范围内，建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择： 如果实验中检测单一指标：不同荧光标记在不同的仪器上强度不同。以某仪器为例：PE ＞APC ＞PE-Cy5 ＞PerCP ＞FITC ＞PerCP-Cy5.5，通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原，使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
