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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD88 (C5aR)
- 级别:
CD88 (C5aR) Antibody
- 目录编号:
CD88 (C5aR) Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD88 (C5aR) Antibody
- 抗体英文名:
Cyanine7 anti-mouse CD88 (C5aR) Antibody-PE
- 抗体名:
CD88 (C5aR) Antibody
- 标记物:
PE
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD88 (C5aR) Antibody
- 亚型:
CD88 (C5aR) Antibody
- 形态:
CD88 (C5aR) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 ug/100 ug
| 规格: | 25 ug | 产品价格: | ¥2200.0 |
|---|---|---|---|
| 规格: | 100 ug | 产品价格: | ¥5500.0 |
Description :CD88 is a single chain protein with seven membrane-spanning regions and a molecular weightof 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have beenreports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells,cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteinssuch as G, and after C5a binding, the signal transduced by CD88 results in the activation ofPLCβ, PI-3 kinase, and PLA , among other molecules, resulting in chemotaxis, granule enzymerelease, and superoxide anion production.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :mC5aR transfected cells
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 underoptimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Godau J, et al. 2004. J. Immunol. 173:3437.
2. Shushakova N, et al. 2002. J. Clin. Invest. 110:1823.
3. Kim SH, et al. 2013. Int Immunol. 25:623. PubMed
Product Citations:
1. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
CD88 分子 CD88 常用单克隆抗体或代号: S5/1,W17/1;(C5aR) 主要表达细胞: My [M] 分子质量(kDa)和结构: gp40 功 能: 补体C5a受体,刺激脱颗粒 CD88 Summary: receptor for C5a, the chemotactic and inflammatory peptide anaphylatoxin
条带。 五、 二抗的选择 二抗应选用与使用的一抗相同的物种来源,例如:如果你的一抗是小鼠的单克隆抗体,二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用, 二抗一般连接荧光素FITC 或发光团。 六、双重染色抗体的选择 用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一,二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 七、荧光和化学发光标记 连接于二抗的标记











