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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD3
- 级别:
CD3 Antibody
- 目录编号:
CD3 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD3 Antibody
- 抗体英文名:
Alexa Fluor® 488 anti-rat CD3 Antibody
- 抗体名:
CD3 Antibody
- 标记物:
CD3 Antibody
- 宿主:
小鼠
- 适应物种:
大鼠
- 免疫原:
CD3 Antibody
- 亚型:
CD3 Antibody
- 形态:
CD3 Antibody
- 应用范围:
FC, IHC-F
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
100 ug
Description :CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of theimmunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressedon thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 isinvolved in antigen recognition, signal transduction, and T cell activation.
Verified Reactivity :Rat
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488under optimal conditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested、IHC-F - Verified
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10 cells in100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration rangeof 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimalperformance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
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文献和实验1. Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP)
2. Nicholls MR, et al. 1993. Transplantation 55:459. (Block)
3. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF)
4. Baba T, et al. 2006. Blood 107:2004. (IHC)
5. Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P)
6. Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed
Product Citations:
1. Wang Y, et al. 2021. Front Cell Dev Biol. 9:702890. PubMed
常用抗体标记荧光染料的选择 随着免疫荧光技术的不断发展,荧光染料及其标记的抗体偶联物也被广泛的应用于生物学实验中。目前,市场上抗体及蛋白标记的荧光染料主要有CFTM系列(BIOTIUM, USA); Alexa Fluor®系列 (Life technology, USA); DyLight系列;Cy系列; IR Dye系列等等。使用最多的为AlexaFluor®系列和CFTM系列。CFTM系列染料的核心对比AlexaFluor®系列具有以下几点优势:1. 新型罗丹明核心罗丹明染料以优异
- 3) 及 CD45 -PE (HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞以 DAPI 染色先行排除,左右分别为分选前后的细胞群分布。 总结: 几次实验的结果,使用 Fab-TACS® Gravity column 来从全血直接进行 CD3+ T 细胞分选,平均得率为 84.5%,纯度大于 90%,细胞存活率高于 95%,且目标细胞不含标记,贴近天然,使下游实验更精确。
及释放目标细胞。以不具磁性且可逆的试剂,取代一般具高亲和力的完整抗体,从而达到直接由全血或其他样本,进行温和标准化正选的目的。Fab-TACS 正选,化解了传统使用完整抗体进行正选的困境,其对目标细胞无不必要的刺激,避免了分选流程的细胞活化,也避免完整抗体与特定受体的结合而影响的分析准确度。因此,使用 Fab-TACS 无痕正选技术可富集不含标记、未激活的目标细胞,次次精准。人全血 CD3+ T 细胞分选实例1. 所需产品:(1) CD3 Fab-TACS® Gravity Kit, human











