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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT/2-8℃/-20℃
- 保质期:
3年
- 英文名:
NON-Fat Powdered Milk
- 库存:
999
- 供应商:
北京康瑞纳生物科技有限公司
- CAS号:
1501-055-9202
- 规格:
100g
脱脂奶粉 Skim Milk (进口分装) 脱脂奶粉 Skim Milk (进口分装)
品名称:脱脂奶粉 Skim Milk (进口分装)
产品编号:A1789
中文别称:脱脂奶粉;
英文别称 NON-Fat Powdered Milk
储存条件 2-25℃
有效期 5年
纯度 Purity≥99.0%
性状 类白色或黄色
规格25g100g 500g
用于制备微生物培养基;配制ELISA、western等实验的封闭液
Skim Milk Skim Milk
Product Name: Skim Milk
Product number: A1789
Chinese nickname: skimmed milk powder;
Also known as NON Fat Powder Milk in English
Storage conditions 2-25 ℃
Validity period of 5 years
Purity ≥ 99.0%
Characteristic white or yellow
Specification: 25g, 100g, 500g
Used for preparing microbial culture media; Prepare a blocking solution for ELISA, Western and other experiments
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文献和实验Ren L, Liu J, Liu C, Yang T, Wu X, Zhang X, Yang L, Xia J, Li W. AIn Vitro Cell(RAW264.7 cells;100 ng/mL LPS,24 h at 37 °C) RAW264.7 cells were seeded in confocal dishes at a density of 1 × 105 cells per dish. After the culture of 24 h, cells were treated with 100 ng/mL LPS in different media, including a control medium, PACDFe extract, and PACDFe hydrogel extract supplemented with 1 μM LL-37 for another 24 h at 1546 °C. 更多文献信息请详询客服
after the final wash, as above. 3.Pipette 100 µl of 5% skim milk/PBS/0.005% thimerasol (added to retard bacterial growth) to each well. Incubate the plate, covered with Parafilm, at 37°C for 2 hours to overnight. It is convenient during this incubation step
on that material are also appropriate. MRS media Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6) Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2) Erythromycin
Blocking of unwanted non-specific staining
4- Incubate with skim milk 5 in TBS-T for 10-30 min. (Miller RT et al, Appl. Immunohist & Molecular Morphology, 71(1): 63-65, 1999) NOTE: skim milk may contain weak proteases which will be useful to further unmask antigens, but may reduce immunostaining










