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- 文献和实验
- 技术资料
- 保存条件:
RT/2-8℃/-20℃
- 保质期:
3年
- 英文名:
Driselase
- 库存:
999
- 供应商:
北京康瑞纳生物科技有限公司
- CAS号:
85186-71-6
- 规格:
1g
崩溃酶 85186-71-6 崩溃酶 85186-71-6
产品名称:崩溃酶 85186-71-6
产品编号:A1881
中文别称 二缩脲;崩溃酶
英文别称 Driselase from Basidiomycetes sp.
CAS 85186-71-6
储存条件 -20℃
有效期 2年
溶解性 1 mg/mL in water
来源 From Basidiomycetes sp.
纯度 Protein approx.10% by Biuret
性状(性状) 浅褐色至黄褐色粉末
包装 支
规格 500mg 1g 5g
说明:崩溃酶是一种粗制酶,主要含纤维素酶和果胶酶。可以水解细胞壁(主要是真菌细胞壁),制备原生质体等。
蛋白含量:≥10%(双缩脲)
酶活定义:37℃、pH4.5下,1单位的崩溃酶1min可以催化1.0mg的还原糖(木聚糖),酶活≥3u/g。
将0.2g本品放入锥形瓶中,溶于10mL 0.4M甘露醇溶液中,用0.1M HCL调节pH至5.2。将1g培养细胞悬浮于溶液中。当酶与细胞在30℃的水谷中轻轻摇晃烧瓶1-2h后,大部分用作材料的细胞将失去细胞壁而变成球形原生质体。如果将培养的细胞用纱布或者尼龙网过滤,在使用前除去大块细胞或调节反应条件,如温度、pH、酶的用量或甘露醇溶液的浓度等,可以改变原生质体制备的比例和速度 。
Explanation: Collapsase is a crude enzyme that mainly contains cellulase and pectinase. It can hydrolyze cell walls (mainly fungal cell walls) and prepare protoplasts.
Protein content: ≥ 10% (biuret)
Enzyme activity definition: At 37 ℃ and pH 4.5, 1 unit of breakdown enzyme can catalyze 1.0mg of reducing sugar (xylan) for 1 minute, with enzyme activity ≥ 3u/g.
Put 0.2g of this product into a conical flask and dissolve it in 10mL of 0.4M mannitol solution. Adjust the pH to 5.2 with 0.1M HCL. Suspend 1g of cultured cells in solution. After gently shaking the flask with enzymes and cells in water at 30 ℃ for 1-2 hours, most of the cells used as materials will lose their cell walls and become spherical protoplasts. If the cultured cells are filtered with gauze or nylon mesh, and large blocks of cells are removed or reaction conditions such as temperature, pH, enzyme dosage, or concentration of mannitol solution are adjusted before use, the proportion and speed of protoplast preparation can be changed.
溶解性:DMSO,鉴定方法:液相,熔点:102℃-260℃,沸点:522.2°C at 760 mmHg,密度:1.064 g/cm³,保存条件:-20℃/2-8℃/RT避光,运输条件:2-8℃,保质期:2年,产地:国产/进口,品牌:北京康瑞纳生物,类别:生化试剂,库存:999,用途:用于科研实验,细胞培养,体外诊断,配方原料,医药生产等方面;使用方法: 详询客服







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