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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD314 (NKG2D)
- 级别:
CD314 (NKG2D) Antibody
- 目录编号:
CD314 (NKG2D) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD314 (NKG2D) Antibody
- 抗体英文名:
Cyanine5 anti-human CD314 (NKG2D) Antibody-PE
- 抗体名:
CD314 (NKG2D) Antibody
- 标记物:
PE
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CD314 (NKG2D) Antibody
- 亚型:
CD314 (NKG2D) Antibody
- 形态:
CD314 (NKG2D) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
CD314 (NKG2D) Antibody
- 规格:
25 tests/ 100 tests
| 规格: | 25 tests | 产品价格: | ¥2700.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥6300.0 |
Description :CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NKcells, CD8 T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have beenidentified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA),MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, butattains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being aprimary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCRmediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligandsplays a role in the immune surveillance against pathogen and tumor cells, and in thepathogenesis of autoimmune diseases.
Verified Reactivity :Human
Reported Reactivity :African Green, Baboon, Cynomolgus, Rhesus
Antibody Type :Monoclonal
Host Species :Mouse
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent betitrated for optimal performance for each application.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Wu J, et al. 1999. Science 285:730.
2. Wu J, et al. 2000. J. Exp. Med. 192:1059.
3. Groh V, et al. 2001. Nature Immunol. 2:255.
4. Wu J, et al. 2002. J. Immunol. 169:1236.
5. Roberts A, et al. 2001. J. Immunol. 167:5527.
6. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
7. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
8. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
9. Watanbe M, et al. 2014. Int Immunol. PubMed
流式课堂 | 易被忽略的 CD4、CD8 共表达细胞——胸腺淋巴细胞
做过流式实验的同学,对 CD4、CD8 这两个指标都不会陌生。检测 CD4+ T 细胞和 CD8+ T 细胞分型,是流式中最常见的实验之一。 通常,我们会先检测 CD3,来圈总T淋巴细胞,再分别用 CD4 抗体和 CD8 抗体,来区分 CD4+ T 淋巴细胞(辅助或调节性T细胞)和 CD8+ T 淋巴细胞(细胞毒性 T 细胞)。 新生小鼠脾脏细胞 上图为新生小鼠脾脏细胞进行的 CD3-PE、CD4-FITC、CD8-APC 三色流式实验结果。从图中可以看出,在新生小鼠脾脏中,绝大
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- 3) 及 CD45 -PE (HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞以 DAPI 染色先行排除,左右分别为分选前后的细胞群分布。 总结: 几次实验的结果,使用 Fab-TACS® Gravity column 来从全血直接进行 CD3+ T 细胞分选,平均得率为 84.5%,纯度大于 90%,细胞存活率高于 95%,且目标细胞不含标记,贴近天然,使下游实验更精确。










