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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
beta Actin抗体
- 抗体英文名:
beta Actin antibody
- 浓度:
1.4 mg/ml (Please refer to the vial label for the specific concentration.)
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, IP
- 宿主:
Rabbit
- 供应商:
欣博盛生物
- 库存:
99
- 保质期:
请参考说明
- 适应物种:
Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Monkey, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Mosquito, Rice, Xenopus, Treeshrew, Plant, Worm
- 克隆性:
多克隆
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 形态:
Liquid
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.
- 规格:
100μl
品牌: GeneTex
产品货号: GTX109639
规格: 100μl
beta-Actin antibody detects beta-actin, a cytoskeletal protein with a predicted molecular weight of 42 kDa. Because of its widespread expression, beta-actin is commonly used as a loading control for various protein assays, particularly western blots, to estimate sample protein amount and quality.
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文献和实验Application Reference
W Tan et al. Neural Regen Res 2024; 19 (1) : 171-179 Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice实验应用 : WB
种属反应 : Mouse
Yoshida K et al. Sci Rep 2023; 13 (1) : 15574 Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells.
Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Chen J et al. J Virol 2023; 97 (3) : e0174322 Identification of a Novel Interferon Lambda Splice Variant in Chickens.
Y Akimoto et al. J Reprod Dev 2023; The effect of ACVR1B/TGFBR1/ACVR1C signaling inhibition on oocyte and granulosa cell development during in vitro growth culture
实验应用 : WB
Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding buffer, spin down 4-8000 rpm
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
buffer. Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding
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