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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
beta Actin抗体
- 抗体英文名:
beta Actin antibody
- 浓度:
1.4 mg/ml (Please refer to the vial label for the specific concentration.)
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, IP
- 宿主:
Rabbit
- 供应商:
欣博盛生物
- 库存:
99
- 级别:
科研用
- 保质期:
请参考说明
- 适应物种:
Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Monkey, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Mosquito, Rice, Xenopus, Treeshrew, Plant, Worm
- 克隆性:
多克隆
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 形态:
Liquid
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.
- 规格:
100μl
beta Actin antibody
品牌: GeneTex
产品货号: GTX109639
规格: 100μl
beta-Actin antibody detects beta-actin, a cytoskeletal protein with a predicted molecular weight of 42 kDa. Because of its widespread expression, beta-actin is commonly used as a loading control for various protein assays, particularly western blots, to estimate sample protein amount and quality.
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.45 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1949572
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
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文献和实验Application Reference
W Tan et al. Neural Regen Res 2024; 19 (1) : 171-179 Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice
实验应用 : WB
种属反应 : Mouse
Yoshida K et al. Sci Rep 2023; 13 (1) : 15574 Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells.
Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Chen J et al. J Virol 2023; 97 (3) : e0174322 Identification of a Novel Interferon Lambda Splice Variant in Chickens.
Y Akimoto et al. J Reprod Dev 2023; The effect of ACVR1B/TGFBR1/ACVR1C signaling inhibition on oocyte and granulosa cell development during in vitro growth culture
实验应用 : WB
Acetylshikonin induces ferroptosis via the lipid peroxidation pathway in osteosarcoma cells.
In Molecular Medicine Reports on 1 February 2026 by Chen, J. Y., Wang, G. S., et al.
Effect of Maternal Hyperglycemia on Cortical Neuronal Migration: Hypofunction of Reelin Signaling.
In Journal of Integrative Neuroscience on 23 January 2026 by Valle-Bautista, R., Soto-Villanueva, I., et al.
beta actin is a type of cell skeleton protein.Its gene is a house keeping gene.In most of cells,beta actin,such as human skeleton,is expressed in a certain level.THat is the reason that people use beta-actin as the criteria of cell numbers
Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding buffer, spin down 4-8000 rpm
-time PCR时,本人不懂操作,将β-actin内参设置成target了,现在跑完了,有扩增曲线图,和融解曲线,但没法导出Ct值(SDS 1.4版本的软件), 请教大虾们有没有补救办法,还是只能重新跑了? 黑色的就是我的beta-actin内参 A2: 重新设置一下不行吗,荧光信号已经收集了。不用重跑。 Q3: 最近在做RT-PCR,但始终摸不清β- actin的意义。哪位可以详细介绍一下β- actin作为内参照的意义?它与目的核酸片段的对比能说明什么问题?谢谢
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