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beta Actin antibody / GTX10963

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  • ¥4000
  • GeneTex已认证
  • GTX109639
  • 美国
  • 2025年07月08日
  • WB, ICC/IF, IHC-P, IHC-Fr, IP
  • Rabbit
  • Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Monkey, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Mosquito, Rice, Xenopus, Treeshrew, Plant, Worm
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      beta Actin抗体

    • 抗体英文名

      beta Actin antibody

    • 浓度

      1.4 mg/ml (Please refer to the vial label for the specific concentration.)

    • 应用范围

      WB, ICC/IF, IHC-P, IHC-Fr, IP

    • 宿主

      Rabbit

    • 供应商

      欣博盛生物

    • 库存

      99

    • 保质期

      请参考说明

    • 适应物种

      Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Monkey, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Mosquito, Rice, Xenopus, Treeshrew, Plant, Worm

    • 克隆性

      多克隆

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 形态

      Liquid

    • 免疫原

      Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

    • 规格

      100μl

    beta Actin antibody
    品牌: GeneTex
    产品货号: GTX109639
    规格: 100μl

    beta-Actin antibody detects beta-actin, a cytoskeletal protein with a predicted molecular weight of 42 kDa. Because of its widespread expression, beta-actin is commonly used as a loading control for various protein assays, particularly western blots, to estimate sample protein amount and quality.

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    图标文献和实验
    相关实验
    • Actin Capture Assay

      Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding buffer, spin down 4-8000 rpm

    • Isolation of Non-muscle Actin

      by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions

    • Actin Capture Assay

      buffer.   Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding

    图标技术资料

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    文献支持
    beta Actin antibody / GTX109639
    ¥4000